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The mechanism of action of GTP on Ca2+ efflux from rat liver microsomal vesicles. Measurement of vesicle fusion by fluorescence energy transfer.
1. GTP-promoted fusion between microsomal vesicles was studied by using fluorescence-resonance-energy transfer between the fluorescent membrane probes octadecanoyl-aminofluorescein and octadecyl-rhodamine. 2. The fluorescence increase after GTP addition does not require the presence of ATP, is unaff...
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Main Authors: | , |
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Format: | Artigo |
Sprog: | Inglês |
Udgivet: |
1988
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Fag: | |
Online adgang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC1148670/ https://ncbi.nlm.nih.gov/pubmed/3342018 |
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