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Chemical synthesis and papain-catalysed hydrolysis of N-alpha-benzyloxycarbonyl-L-lysine p-nitroanilide.

The chemical synthesis of N-alpha-benzyloxycarbonyl-L-lysine p-nitroanilide (Z-Lys-pNA) is described in detail. The pH-dependence of the catalytic parameters kcat,' Km and kcat./Km for the papain-catalysed hydrolysis of Z-Lys-pNA are determined. kcat. and Km are pH-independent between pH 5 and...

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Bibliografiske detaljer
Main Authors: Mackenzie, N E, Malthouse, J P, Scott, A I
Format: Artigo
Sprog:Inglês
Udgivet: 1985
Fag:
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC1144749/
https://ncbi.nlm.nih.gov/pubmed/3922354
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