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Chemical synthesis and papain-catalysed hydrolysis of N-alpha-benzyloxycarbonyl-L-lysine p-nitroanilide.
The chemical synthesis of N-alpha-benzyloxycarbonyl-L-lysine p-nitroanilide (Z-Lys-pNA) is described in detail. The pH-dependence of the catalytic parameters kcat,' Km and kcat./Km for the papain-catalysed hydrolysis of Z-Lys-pNA are determined. kcat. and Km are pH-independent between pH 5 and...
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Main Authors: | , , |
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Format: | Artigo |
Sprog: | Inglês |
Udgivet: |
1985
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Fag: | |
Online adgang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC1144749/ https://ncbi.nlm.nih.gov/pubmed/3922354 |
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