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A rapid method for efficient gene replacement in the filamentous fungus Aspergillus nidulans

The construction of mutant fungal strains is often limited by the poor efficiency of homologous recombination in these organisms. Higher recombination efficiencies can be obtained by increasing the length of homologous DNA flanking the transformation marker, although this is a tedious process when s...

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Autori principali: Chaveroche, Marie-Kim, Ghigo, Jean-Marc, d’Enfert, Christophe
Natura: Artigo
Lingua:Inglês
Pubblicazione: Oxford University Press 2000
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Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC113889/
https://ncbi.nlm.nih.gov/pubmed/11071951
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