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Purification and Characterization of Leu-Proteinase, the Leucine Specific Serine Proteinase from Spinach (Spinacia oleracea L.) Leaves

The leucine specific serine proteinase present in the soluble fraction of leaves from Spinacia oleracea L. (called Leu-proteinase) has been purified by acetone precipitation and a combination of gel-filtration, ion exchange, and adsorption chromatography. This enzyme shows a molecular weight of 60,0...

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Main Authors: Aducci, Patrizia, Ascenzi, Paolo, Pierini, Marco, Ballio, Alessandro
Format: Artigo
Jezik:Inglês
Izdano: 1986
Teme:
Online dostop:https://ncbi.nlm.nih.gov/pmc/articles/PMC1075432/
https://ncbi.nlm.nih.gov/pubmed/16664908
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