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Use of terminal transferase-dependent antisense RNA amplification to determine the transcription start site of the Snrpn gene in individual neurons

We describe here a very sensitive technique for RNA structure analysis and the determination of transcription start sites and demonstrate its use for mapping the start site of the imprinted Snrpn gene in individual hippocampal neurons. The method is adapted from reverse transcription–terminal transf...

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Main Authors: Buettner, Victoria L., LeBon, Jeanne M., Gao, Chunguang, Riggs, Arthur D., Singer-Sam, Judith
格式: Artigo
語言:Inglês
出版: Oxford University Press 2000
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在線閱讀:https://ncbi.nlm.nih.gov/pmc/articles/PMC102808/
https://ncbi.nlm.nih.gov/pubmed/10710442
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