Use of terminal transferase-dependent antisense RNA amplification to determine the transcription start site of the Snrpn gene in individual neurons

We describe here a very sensitive technique for RNA structure analysis and the determination of transcription start sites and demonstrate its use for mapping the start site of the imprinted Snrpn gene in individual hippocampal neurons. The method is adapted from reverse transcription–terminal transf...

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Hlavní autoři: Buettner, Victoria L., LeBon, Jeanne M., Gao, Chunguang, Riggs, Arthur D., Singer-Sam, Judith
Médium: Artigo
Jazyk:Inglês
Vydáno: Oxford University Press 2000
Témata:
NAR Methods Online
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC102808/
https://ncbi.nlm.nih.gov/pubmed/10710442
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