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Purification and Characterization of the DeoR Repressor of Bacillus subtilis

Transcription of the Bacillus subtilis dra-nupC-pdp operon is repressed by the DeoR repressor protein. The DeoR repressor with an N-terminal His tag was overproduced with a plasmid under control of a phage T5 promoter in Escherichia coli and was purified to near homogeneity by one affinity chromatog...

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Autori principali: Zeng, Xianmin, Saxild, Hans H., Switzer, Robert L.
Natura: Artigo
Lingua:Inglês
Pubblicazione: American Society for Microbiology 2000
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Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC101877/
https://ncbi.nlm.nih.gov/pubmed/10714997
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