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Purification and Characterization of the DeoR Repressor of Bacillus subtilis
Transcription of the Bacillus subtilis dra-nupC-pdp operon is repressed by the DeoR repressor protein. The DeoR repressor with an N-terminal His tag was overproduced with a plasmid under control of a phage T5 promoter in Escherichia coli and was purified to near homogeneity by one affinity chromatog...
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Main Authors: | , , |
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Format: | Artigo |
Sprog: | Inglês |
Udgivet: |
American Society for Microbiology
2000
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Fag: | |
Online adgang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC101877/ https://ncbi.nlm.nih.gov/pubmed/10714997 |
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