Visualization of Spirochetes by Labeling Membrane Proteins With Fluorescent Biarsenical Dyes

Numerous methods exist for fluorescently labeling proteins either as direct fusion proteins (GFP, RFP, YFP, etc.—attached to the protein of interest) or utilizing accessory proteins to produce fluorescence (SNAP-tag, CLIP-tag), but the significant increase in size that these accompanying proteins ad...

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Main Authors: Chadwick Hillman, Philip E. Stewart, Martin Strnad, Hunter Stone, Tregei Starr, Aaron Carmody, Tyler J. Evans, Valentina Carracoi, Jenny Wachter, Patricia A. Rosa
格式: Artigo
語言:Inglês
出版: Frontiers Media S.A. 2019-08-01
叢編:Frontiers in Cellular and Infection Microbiology
主題:
spirochetes
Borrelia
Leptospira
fluorescent protein
tetracysteine tag
biarsenical dye
在線閱讀:https://www.frontiersin.org/article/10.3389/fcimb.2019.00287/full
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