Dissociation of solid tumor tissues with cold active protease for single-cell RNA-seq minimizes conserved collagenase-associated stress responses

Abstract Background Single-cell RNA sequencing (scRNA-seq) is a powerful tool for studying complex biological systems, such as tumor heterogeneity and tissue microenvironments. However, the sources of technical and biological variation in primary solid tumor tissues and patient-derived mouse xenogra...

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Autori principali: Ciara H. O’Flanagan, Kieran R. Campbell, Allen W. Zhang, Farhia Kabeer, Jamie L. P. Lim, Justina Biele, Peter Eirew, Daniel Lai, Andrew McPherson, Esther Kong, Cherie Bates, Kelly Borkowski, Matt Wiens, Brittany Hewitson, James Hopkins, Jenifer Pham, Nicholas Ceglia, Richard Moore, Andrew J. Mungall, Jessica N. McAlpine, The CRUK IMAXT Grand Challenge Team, Sohrab P. Shah, Samuel Aparicio
Natura: Artigo
Lingua:Inglês
Pubblicazione: BMC 2019-10-01
Serie:Genome Biology
Soggetti:
Single cell
RNA-seq
Tissue dissociation
Gene expression
Quality control
Breast cancer
Accesso online:http://link.springer.com/article/10.1186/s13059-019-1830-0
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