X-Ray Crystal Structure and Properties of Phanta, a Weakly Fluorescent Photochromic GFP-Like Protein.

Phanta is a reversibly photoswitching chromoprotein (ΦF, 0.003), useful for pcFRET, that was isolated from a mutagenesis screen of the bright green fluorescent eCGP123 (ΦF, 0.8). We have investigated the contribution of substitutions at positions His193, Thr69 and Gln62, individually and in combinat...

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Bibliographic Details
Main Authors: Craig Don Paul, Daouda A K Traore, Seth Olsen, Rodney J Devenish, Devin W Close, Toby D M Bell, Andrew Bradbury, Matthew C J Wilce, Mark Prescott
Format: Artigo
Language:Inglês
Published: Public Library of Science (PLoS) 2015-01-01
Series:PLoS ONE
Online Access:http://europepmc.org/articles/PMC4414407?pdf=render
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