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A new in vitro strand transfer assay for monitoring bacterial class 1 integron recombinase IntI1 activity.

IntI1 integrase is a tyrosine recombinase involved in the mobility of antibiotic resistance gene cassettes within bacterial class 1 integrons. Recent data have shown that its recombination specifically involves the bottom strand of the attC site, but the exact mechanism of the reaction is still uncl...

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Autores principales: Véronique Dubois, Carole Debreyer, Simon Litvak, Claudine Quentin, Vincent Parissi
Formato: Artigo
Lenguaje:Inglês
Publicado: Public Library of Science (PLoS) 2007-01-01
Colección:PLoS ONE
Acceso en línea:http://europepmc.org/articles/PMC2117344?pdf=render
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