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A new in vitro strand transfer assay for monitoring bacterial class 1 integron recombinase IntI1 activity.
IntI1 integrase is a tyrosine recombinase involved in the mobility of antibiotic resistance gene cassettes within bacterial class 1 integrons. Recent data have shown that its recombination specifically involves the bottom strand of the attC site, but the exact mechanism of the reaction is still uncl...
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Hauptverfasser: | , , , , |
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Format: | Artigo |
Sprache: | Inglês |
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Public Library of Science (PLoS)
2007-01-01
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Schriftenreihe: | PLoS ONE |
Online Zugang: | http://europepmc.org/articles/PMC2117344?pdf=render |
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