A unique mono- and diacylglycerol lipase from Penicillium cyclopium: heterologous expression, biochemical characterization and molecular basis for its substrate selectivity.

A cDNA gene encoding a mature peptide of the mono- and diacylglycerol lipase (abbreviated to PcMdl) from Penicillium cyclopium PG37 was cloned and expressed in Pichia pastoris GS115. The recombinant PcMdl (rePcMdl) with an apparent molecular weight of 39 kDa showed the highest activity (40.5 U/mL of...

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Hlavní autoři: Zhong-Biao Tan, Jian-Fang Li, Xue-Ting Li, Ying Gu, Min-Chen Wu, Jing Wu, Jun-Qing Wang
Médium: Artigo
Jazyk:Inglês
Vydáno: Public Library of Science (PLoS) 2014-01-01
Edice:PLoS ONE
On-line přístup:http://europepmc.org/articles/PMC4106778?pdf=render
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