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Assessing mycoplasma contamination of cell cultures by qPCR using a set of universal primer pairs targeting a 1.5 kb fragment of 16S rRNA genes.

Mycoplasmas (a generic name for Mollicutes) are a predominant bacterial contaminant of cell culture and cell derived products including viruses. This prokaryote class is characterized by very small size and lack of a cell wall. Consequently, mycoplasmas escape ultrafiltration and visualization under...

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Main Authors: Audrey Jean, Florence Tardy, Omran Allatif, Isabelle Grosjean, Bariza Blanquier, Denis Gerlier
Format: Artigo
Jezik:Inglês
Izdano: Public Library of Science (PLoS) 2017-01-01
Serija:PLoS ONE
Online dostop:http://europepmc.org/articles/PMC5321415?pdf=render
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