Engineered Cas12i2 is a versatile high-efficiency platform for therapeutic genome editing

Cas12i is a genome editing platform with compact size that fits in AAV vector with short 43-mer gRNA, absence of tracrRNA, ability to process pre-crRNA, and high specificity. Here the authors present an unbiased mutational scanning approach to engineer Cas12i, which shows low activity in mammalian c...

Descripción completa

Guardado en:
Detalles Bibliográficos
Autores principales: Colin McGaw, Anthony J. Garrity, Gabrielle Z. Munoz, Jeffrey R. Haswell, Sejuti Sengupta, Elise Keston-Smith, Pratyusha Hunnewell, Alexa Ornstein, Mishti Bose, Quinton Wessells, Noah Jakimo, Paul Yan, Huaibin Zhang, Lauren E. Alfonse, Roy Ziblat, Jason M. Carte, Wei-Cheng Lu, Derek Cerchione, Brendan Hilbert, Shanmugapriya Sothiselvam, Winston X. Yan, David R. Cheng, David A. Scott, Tia DiTommaso, Shaorong Chong
Formato: Artigo
Lenguaje:Inglês
Publicado: Nature Portfolio 2022-05-01
Colección:Nature Communications
Acceso en línea:https://doi.org/10.1038/s41467-022-30465-7
Etiquetas: Agregar Etiqueta
Sin Etiquetas, Sea el primero en etiquetar este registro!

Ejemplares similares