Agrobacterium-mediated transient transformation of sorghum leaves for accelerating functional genomics and genome editing studies

Abstract Objectives Sorghum is one of the most recalcitrant species for transformation. Considering the time and effort required for stable transformation in sorghum, establishing a transient system to screen the efficiency and full functionality of vector constructs is highly desirable. Results Her...

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Bibliographic Details
Main Authors: Rita Sharma, Yan Liang, Mi Yeon Lee, Venkataramana R. Pidatala, Jenny C. Mortimer, Henrik V. Scheller
Format: Artigo
Language:Inglês
Published: BMC 2020-02-01
Series:BMC Research Notes
Subjects:
Agrobacterium
CRISPR
sgRNA
Sorghum
Transformation
Transient
Online Access:http://link.springer.com/article/10.1186/s13104-020-04968-9
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