Barcode identification for single cell genomics

Abstract Background Single-cell sequencing experiments use short DNA barcode ‘tags’ to identify reads that originate from the same cell. In order to recover single-cell information from such experiments, reads must be grouped based on their barcode tag, a crucial processing step that precedes other...

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Bibliographic Details
Main Authors: Akshay Tambe, Lior Pachter
Format: Artigo
Language:Inglês
Published: BMC 2019-01-01
Series:BMC Bioinformatics
Subjects:
Single-cell
Barcodes
Barcode identification
de Bruijn graph
Circularization
K-mer counting
Online Access:http://link.springer.com/article/10.1186/s12859-019-2612-0
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