Factors influencing electroporation-mediated gene transfer to Stylosanthes guianensis (Aubl.) Sw. protoplasts

In order to develop a high-efficiency and reproducible transformation protocol for Stylosanthes guianensis we assessed the biological and physical parameters affecting plant electroporation protoplasts. Energy input, as combinations of electric field strengths discharged by different capacitors, ele...

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Bibliographic Details
Main Authors: Quecini V.M., Oliveira C.A. de, Alves A.C., Vieira M.L.C.
Format: Artigo
Language:Inglês
Published: Sociedade Brasileira de Genética 2002-01-01
Series:Genetics and Molecular Biology
Subjects:
direct gene transfer
plant transformation
forage legume
Stylosanthes guianensis
Online Access:http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572002000100014
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