Detection of Borrelia-specific 16S rRNA sequence in total RNA extracted from Ixodes ricinus ticks

A reverse transcriptase - polymerase chain reaction based assay for Borrelia species detection in ticks was developed. The method was based on amplification of 552 nucleotide bases long sequence of 16S rRNA, targeted by Borrelia specific primers. In the present study, total RNA extracted from Ixodes...

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Bibliographic Details
Main Authors: Ž. Radulović, M. Milutinović, S. Tomanović, A. Mulenga
Format: Artigo
Language:Inglês
Published: Universidade Federal de Minas Gerais 2010-08-01
Series:Arquivo Brasileiro de Medicina Veterinária e Zootecnia
Subjects:
Borrelia
RT-PCR
16S rRNA
Ixodes ricinus
Online Access:http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352010000400015&lng=en&tlng=en
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