1-aminocyclopropane-1-carboxylic acid oxidase Agaricus sinodeliciosus var. Chaidam CRISPR/Cas9 Flammulina filiformis GCN2 Ganoderma lingzhi Ganoderma lucidum Hypsizygus marmoreus Lentinula edodes RNA sequencing RNA-seq RNPs Tween80 U6 promoter alternative splicing ascomycetes basal ganglia basidiomycete basidiomycetes biological activity cerebellum chimeric gene cortex cultivation dual sgRNAs functional gene analysis ganoderic acid gene editing genome sequencing genomic editing